Stochastic exit from mitosis in budding yeast Model predictions and experimental observations

TitleStochastic exit from mitosis in budding yeast Model predictions and experimental observations
Publication TypeJournal Article
Year of Publication2011
AuthorsBall, D. A., T. - H. Ahn, P. Wang, K. C. Chen, Y. Cao, J. J. Tyson, J. Peccoud, and W. T. Baumann
JournalCELL CYCLE
Volume10
Issue6
Pagination999-1009
Date PublishedMAR 15
Type of ArticleArticle
ISSN1538-4101
Abstract

{Unlike many mutants that are completely viable or inviable, the CLB2-db Delta clb5 Delta mutant of Saccharomyces cerevisiae is inviable in glucose but partially viable on slower growth media such as raffinose. On raffinose, the mutant cells can bud and divide but in each cycle there is a chance that a cell will fail to divide (telophase arrest), causing it to exit the cell cycle. This effect gives rise to a stochastic phenotype that cannot be explained by a deterministic model. We measure the interbud times of wild-type and mutant cells growing on raffinose and compute statistics and distributions to characterize the mutant's behavior. We convert a detailed deterministic model of the budding yeast cell cycle to a stochastic model and determine the extent to which it captures the stochastic phenotype of the mutant strain. Predictions of the mathematical model are in reasonable agreement with our experimental data and suggest directions for improving the model. Ultimately, the ability to accurately model stochastic phenotypes may prove critical to understanding disease and therapeutic interventions in higher eukaryotes.}

DOI10.4161/cc.10.6.14966